The number of BrdU+ and BrdU+/Iba+ cells was quantified on digitalized confocal images captured from six serial 40-μm sections spaced 0.32 mm apart (two fields of view per section; Zeiss LSM 710) in ipsi- and contralateral cortices. Three-dimensional colocalization of protein markers was validated using confocal z-stacks.
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2ff7e9595c
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